RNA
CCA

Part:BBa_K5425002:Design

Designed by: Sampoorn Pandey   Group: iGEM24_IISER-Kolkata   (2024-09-21)


Antisese mRNA against the mRNA of dnaA protein.(x=400BP)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 417
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 418
    Illegal PstI site found at 432
    Illegal NotI site found at 7
    Illegal NotI site found at 425
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 418
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal SpeI site found at 418
    Illegal PstI site found at 432
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to calibrate then length of anti sense which we were taking in order to get the most optimal binding with least non specificity. We also considered the secondary structure of the antisense while choosing the length.These designing were aided by RNAfold software.


Source

This part was taken from the genomic sequence of BL21(DE3) E coli.

References